移动式叶绿素荧光成像系统PlantExplorerXS是由慧诺瑞德和荷兰PhenoVation公司联合推出的专门针对大田、温室、气候室和实验室场景的可以移动的叶绿素荧光测量系统。配备移动式升降平台车、内置电脑的叶绿素荧光成像单元、移动电源、显示单元和操作单元。叶绿素荧光成像单元可以升降和旋转,既可以测量不同高度的植物冠层,也可以倾斜或水平角度测量穗(麦穗、稻穗、谷穗等)、荚果(大豆、油菜等)、果实(番茄、黄瓜、葡萄、柑橘等)、叶片或冠层。
该系统成像面积为18x18cm,具备500万像素高清成像,同时具备“调制”和“非调制”叶绿素荧光成像测量功能,既可以测量光合生理,也可以测量形态结构,同时配备功能强大的控制和分析软件,且可以对大量数据进行批处理分析。该系统,无论室内还是大田,都是进行植物表型、光合生理、植物抗逆、植物病理、育种、功能基因组、突变株筛选、种子生理/病理等研究的利器。
功能特性
- 大田、温室、气候室、实验室进行移动式测量
- 叶绿素荧光成像单元可以升降、旋转
- 叶绿素荧光成像和表型分析同步测量
- 同时具备调制和非调制叶绿素荧光测量功能
- 出色的高清相机(500万像素)、高信噪比成像
- 16位图像格式,的成像质量
- 光源、相机、滤光片、电脑一体化设计
- 无可见镜头畸变,无需图像校正
- 成像范围18 x 18cm
- 多种测量protocol可选,允许用户编辑设定自己的protocol,包括但不限于Fv/Fm测量、标准诱导曲线测量、暗弛豫测量、OJIP快速诱导动力学测量等等。
- 可进行功能强大的延时成像测量
- 自动计算荧光参数和表型参数
- 具备图像数据批处理分析功能
- 提供多种功能强大的图像分割功能
- 对所有图像数据均提供数据分级(用户自定义范围)并进行图像化显示,并允许对分级筛选后的数据叠加到可见光图像上展示
- 图像背景、伪彩色标尺均有多种选择
- 允许用户自定义多种ROI(性状、数目、分布等)并对ROI的数据自动分析
- 嵌入式电脑进行精确的成像、时间控制、光强控制和数据存储
- 功能强大的控制和分析软件
- 特别适合突变株筛选、育种材料/组合筛选、抗逆研究、病理研究、种子研究、果实研究、功能基因组学等
主要技术参数
- 基本组成:移动式升降平台、叶绿素荧光成像单元、移动电源、显示单元、操作单元等
- 叶绿素荧光成像方式:“调制”测量 +“费调制”测量
- 调制测量光:蓝色LED, 450nm,半峰全宽20nm,光强4000 umol m-2 s-1 ,独立触发
- Kautsky测量光:蓝色LED, 450nm,半峰全宽20nm,光强4000 umol m-2 s-1
- 饱和脉冲:蓝色LED, 450nm,半峰全宽20nm,光强4000 umol m-2 s-1,独立触发
- 时间分辨动力学光化光:红光LED,660nm,光强800 umol m-2 s-1
- 远红光:LED,735nm,半峰全宽20nm,35W
- 相机:CMOS传感器,500万像素
- 颜色深度:12bit
- 标准帧率:37.5 FPS
- 图像格式:16bit
- 相机光谱范围:400~1000 nm
- 接口:3个USB3.0,1个以太网口,1个HDMI接口
- 嵌入式电脑:4核处理器,8G内存,256G固态硬盘
- 成像面积:18cm x 18cm
- 升降高度:0-1200mm(高度可定制)
- 旋转角度:-90° ~ 90°
- 显示单元:15.6寸触摸显示屏
- 供电:35万mAh移动电源,额定容量1260Wh,峰值功耗1000W,待机功耗35W
- 系统尺寸:600mm x 720mm x 2000mm(长x宽x高)
测量参数
- 调制叶绿素荧光参数:Fo、Fm、Fv/Fm、dFq/Fm=DF/Fm、Fs’、Fm’、Fo’、Fq’/Fm’=Fv’/Fm’、rETR、NPQ、Y(NO)、Y(NPQ)、qN、qP、qL、1-qP和1-qL等;
- 非调制叶绿素荧光参数:Fo、Fi、Fm、1-Fi/Fm、IC-Area、IC-Area/Fv、PI、Rfd、dRfd、RfdFm和RfdFt等;
- 表型参数:(植物、种子、果实的)数目、轮廓面积、长度、宽度、凸包点数、凸包面积、凸包面积/轮廓面积、最小外接圆(质心、半径、面积)、最小外接矩形(长、宽、面积、角度、alpha)和骨架等。
利用PhenoVation叶绿素荧光成像技术发表的部分文献
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